MICROSATELLITE MARKERS ISOLATED FROM THE WILD MEDICINAL PLANT CENTELLA ASIATICA (APIACEAE) FROM AN ENRICHED GENOMIC LIBRARY

Abstract : Premise of the study: Microsatellite markers for Centella asiatica , an important medicinal herb, were developed and character- ized to promote genetic and molecular studies. * Methods and Results: A GA/GT-enriched genomic library was constructed from an accession from Madagascar. Roughly 75% of the 768 clones of the enriched library contained microsatellites. Eighty sequences containing microsatellites were obtained from 96 positive clones. Specifi c primers were designed for 20 loci, and 17 of them displayed polymorphism when screened across 17 C. asiatica accessions, with an average of 4.3 alleles per locus . The observed and expected heterozygosity values averaged 0.114 and 0.379, respectively. * Conclusions: This is the fi rst report constructing an enriched genomic library and identifying microsatellite markers from C. asiatica . These 17 polymorphic microsatellite markers are a useful resource for this plant, applicable for diversity studies, pedigree analyses, and genetic mapping.
Document type :
Journal articles
Complete list of metadatas

http://hal.cirad.fr/cirad-00826834
Contributor : Mada Webcirad <>
Submitted on : Saturday, May 31, 2014 - 7:00:04 AM
Last modification on : Monday, November 18, 2019 - 3:29:18 PM
Long-term archiving on: Sunday, August 31, 2014 - 10:36:10 AM

File

Rakondralambo_et_al.pdf_2012.p...
Files produced by the author(s)

Identifiers

  • HAL Id : cirad-00826834, version 1

Citation

Soaharin'Ny Ony Raoseta Rakotondralambo, Alexadre Lussert, Ronan Rivallan, Pascal Danthu, Jean-Louis Noyer, et al.. MICROSATELLITE MARKERS ISOLATED FROM THE WILD MEDICINAL PLANT CENTELLA ASIATICA (APIACEAE) FROM AN ENRICHED GENOMIC LIBRARY. American Journal of Botany, Botanical Society of America, 2013, pp.e176-e178. ⟨cirad-00826834⟩

Share

Metrics

Record views

693

Files downloads

464