Microsatellite Primer Amplification by Multiplexing: A First Application to Eucalyptus grandis

Gilles Chaix 1 Isabelle Chantal 2 Mireille Poitel 1 Sylvie Razafiarivelo 1 Daniel Verhaegen 1 Jean Charles Maillard 2
1 CIRAD-FORET - CIRAD-Département Forêt
2 CIRAD-EMVT - CIRAD- Département Elevage et médecine vétérinaire
Abstract : Multiplexing was used to increase the efficiency of microsatellite analysis in Eucalyptus grandis. Primers were chosen based on the range of eucalyptus SSR alleles and their hybridization temperatures. Choice of PCR conditions proved to be the most impor- tant factor; concentrations of primers must be adjusted for use in combination. Results show that 6 primers can be analyzed in 2 PCR reactions, one containing 2 and the other containing 4 primers. Multiplexing promises to be a particularly efficient tool for micro- satellite diversity analysis in Eucalyptus grandis
keyword : Eucalyptus grandis microsatellite multiplexing primer sequencer
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Journal articles
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Submitted on : Wednesday, July 17, 2013 - 3:06:17 PM
Last modification on : Friday, March 29, 2019 - 9:11:20 AM

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Gilles Chaix, Isabelle Chantal, Mireille Poitel, Sylvie Razafiarivelo, Daniel Verhaegen, et al.. Microsatellite Primer Amplification by Multiplexing: A First Application to Eucalyptus grandis. Plant Molecular Biology Reporter, Springer Verlag, 2002, 20, pp.67a-67e. ⟨cirad-00845657⟩

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