Gene flow estimation with microsatellites in a Malagasy seed orchard of Eucalyptus grandis

G. Chaix 1 S. A. Gerber 2 V. Razafimaharo 1 P. Vigneron 1 D. Verhaegen 1 S. Hamon 3
1 CIRAD-FORET - CIRAD-Département Forêt
2 INRA, Laboratoire de gé nétique et amé lioration des arbres forestiers
3 IRD Montpellier
Abstract : Eucalyptus grandis has a mixed-mating repro- ductive system. Malagasy Eucalyptus seed orchards were established 15 years ago with two aims both based on panmixia: open-pollinated seed production and genetic improvement. The panmixia hypothesis has never been confirmed in the seed orchard. From a seedling seed- orchard stand comprising 349 trees and using data obtained with six selected microsatellite markers, pater- nity analysis was performed for 724 offspring collected on 30 adult trees. Paternity assignment, based on exclu- sion procedures and likelihood-ratio method, was achieved with high accuracy; the exclusion probability value was 0.997. The outcrossing rate was very high (96.7%). More than 50% of potential male trees (199 out of 349) in the seed orchard contributed to pollination for 440 offspring of 30 progenies (8.6% of the basic population). The pollination rate from outside the seed orchard was high (39.2%), but might be due to the small size of this seed orchard. This study showed that "panmixia-like pollination" can be assumed
Keywords : Madagascar Microsatellite Seed orchard Paternity analysis Pollen flow Eucalyptus grandis
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G. Chaix, S. A. Gerber, V. Razafimaharo, P. Vigneron, D. Verhaegen, et al.. Gene flow estimation with microsatellites in a Malagasy seed orchard of Eucalyptus grandis. TAG Theoretical and Applied Genetics, Springer Verlag, 2003, 107, pp.705-712. ⟨cirad-00846100⟩

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