EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis

Monica Goicoechea 1 Eric Lacombe 1 Sylvain Legay 1 Csnjezana Mihaljevi 1 Philippe Rech 1 Alain Jauneau 1 Catherine Lapierre 2 Brigitte Pollet 2 Daniel Verhaegen 3 Nicole Chaubet-Gigot 1 Jacqueline Grima-Pettenati 1
1 Institut Fe 'de ' ratif de Recherches FR40, Unité mixte de Recherches 5546, Université Paul Sabatier-Centre National de la Recherche Scientifique, Pôle de Biotechnologie Végétale
2 UCB - Chimie Biologique
3 CIRAD-FORET - CIRAD-Département Forêt
Abstract : EgMYB2, a member of a new subgroup of the R2R3 MYB family of transcription factors, was cloned from a library consisting of RNA from differentiating Eucalyptus xylem. EgMYB2 maps to a unique locus on the Eucalyptus grandis linkage map and co-localizes with a quantitative trait locus (QTL) for lignin content. Recombinant EgMYB2 protein was able to bind specifically the cis-regulatory regions of the promoters of two lignin biosynthetic genes, cinnamoyl-coenzyme A reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD), which contain MYB consensus binding sites. EgMYB2 was also able to regulate their transcription in both transient and stable expression assays. Transgenic tobacco plants over-expressing EgMYB2 displayed phenotypic changes relative to wild-type plants, among which were a dramatic increase in secondary cell wall thickness, and an alteration of the lignin profiles. Transcript abundance of genes encoding enzymes specific to lignin biosynthesis was increased to varying extents according to the position of individual genes in the pathway,whereas core phenylpropanoid geneswere not significantly affected. Together these results suggest a role for EgMYB2 in the co-ordinated control of genes belonging to the monolignol-specific pathway, and therefore in the biosynthesis of lignin and the regulation of secondary cell wall formation.
Keywords : MYB transcription xylem lignin secondary cell wall
Document type :
Journal articles
Complete list of metadatas

Cited literature [43 references]  Display  Hide  Download
http://hal.cirad.fr/cirad-00846177
Contributor : Mada Webcirad <>
Submitted on : Thursday, July 18, 2013 - 4:23:48 PM
Last modification on : Tuesday, August 27, 2019 - 9:20:05 AM
Long-term archiving on: Monday, October 21, 2013 - 9:55:15 AM

File

The_plant_Journal_43_4_.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : cirad-00846177, version 1

Collections

CIRAD | FORET-BIO | INRA | AGROPARISTECH | UNIV-TLSE3

Citation

Monica Goicoechea, Eric Lacombe, Sylvain Legay, Csnjezana Mihaljevi, Philippe Rech, et al.. EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis. Plant Journal, Wiley, 2005, 43, pp.553-567. ⟨cirad-00846177⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

627

Files downloads

673

Contact

Informations

CCSD