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Boosting the sterile insect technique with pyriproxyfen increases tsetse flies Glossina palpalis gambiensis sterilization in controlled conditions

Abstract : Tsetse flies (Diptera: Glossinidae) are the main vectors of animal and human trypanosomoses in Africa. The Sterile Insect Technique (SIT) has proven effective in controlling tsetse flies when applied to isolated populations but necessitates the production of large numbers of sterile males. A new approach, called boosted SIT, combining SIT with the contamination of wild females by sterile males coated with biocides has been proposed for large-scale control of vector populations. The aim of the study was to evaluate this new approach using pyriproxyfen on the riverine species Glossina palpalis gambiensis (Vanderplank, 1949) in the laboratory. The contamination dose and persistence of pyriproxyfen on sterile males, the impact of pyriproxyfen on male survival, and the dynamics of pyriproxyfen transfer from a sterile male to a female during mating, as well as the impact of pyriproxyfen on pupal production and adult emergence, were evaluated in the laboratory. For this purpose, a method of treatment by impregnating sterile males with a powder containing 40% pyriproxyfen has been developed. The results showed that the pyriproxyfen has no impact on the survival of sterile males. pyriproxyfen persisted on sterile males for up to 10 days at a dose of 100 ng per fly. In addition, the horizontal transfer of pyriproxyfen from a treated sterile male to a female during mating could be measured with an average of 50 ng of pyriproxyfen transferred. After contacts without mating, the average quantity transferred was more than 10 ng. Finally, the pyriproxyfen powder was very effective on G. p. gambiensis leading to 0% emergence of the pupae produced by contaminated females. These promising results must be confirmed in the field. A large-scale assessment of this boosted pyriproxyfen-based SIT approach will be carried out against tsetse flies in Senegal (West Africa). On the African continent, tsetse flies (Diptera: Glossinidae) are the main vectors of the parasites responsible for Human African Trypanosomosis (HAT), or sleeping sickness, and Animal African Trypanosomosis (AAT), also called Nagana 1. These diseases caused by Trypanosomatidae belong to the group of neglected tropical diseases (NTDs), occurring in developing areas 2. Sub-Saharan African countries suffer from a significant impact of NTDs on public health and economic development 3. Glossina palpalis gambiensis (Vanderplank, 1949) is one of the main vectors of the HAT parasite in West Africa and is responsible for the persistence of many residual outbreaks of this disease in forest and savannah areas 4. There is still no vaccine against HAT and curative treatments are difficult to access for the most exposed and vulnerable populations 5. Glossina palpalis gambiensis is also involved in the transmission of AAT, which affects livestock and reduces animal production thus limiting the availability of food resources 6,7. Despite the millions of doses of trypanocides administered, nearly 3 million cattle die each year in Africa from AAT. Annual direct and indirect agricultural losses attributed to this disease are estimated at
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Soumis le : mercredi 24 juin 2020 - 11:41:44
Dernière modification le : lundi 24 août 2020 - 16:12:23

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L Laroche, S Ravel, T. Baldet, R. Lancelot, F. Chandre, et al.. Boosting the sterile insect technique with pyriproxyfen increases tsetse flies Glossina palpalis gambiensis sterilization in controlled conditions. Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-020-66850-9⟩. ⟨cirad-02879794⟩

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